Typical Treatment
– IVF 2
3. Egg Retrieval & Sperm Collection
Egg Retrieval:
Eggs are contained within follicular fluid. Egg retrieval is accomplished with the use of transvaginal ultrasound. Transvaginal ultrasound is used to determine the position of the ovarian follicle and to help guide a collection needle to the site of each follicle. Each egg and the surrounding follicular fluid are then withdrawn. No pain should be experienced during this procedure since it takes place while under anesthesia. The effects of the anesthesia are strong but short lasting. Patients who have undergone general anesthesia can leave the hospital within 2 hours after collecting the oocytes. If only a few eggs (such as 1 or 2) are to be retrieved or if the eggs are located in an area where you should not experience much pain, a suppository painkiller may be all that is required for pain treatment during egg retrieval.
Sperm Collection:
In order to ensure that your semen is in pristine condition, we ask you to provide a fresh sample of semen at our hospital in the room provided. The semen is then washed with a culture medium and centrifuged so that we may select the most healthy and active sperm from it.
Schedule:
AM 8:00 Arrive at hospita / Checking for oocyte by ultrasound
AM 8:45 Explanation for procedure
AM 9:00 Changing clothes
AM 9:30 Premedication （injection）
AM10:00 Entrance into operating room, sperm collection / General anesthesia, cleansing
AM10:20 Oocyte collection
AM10:40 Return to room
PM11:00 Wake up
PM12:00 Post examination by ultrasound & explanation
PM12:30 Leave the hospital
4. In Vitro Fertilization
After the eggs are retrieved they are cultivated for approximately 4-5 hours, to make either matarity completed then they are then placed together with the selected sperm in a laboratory dish for insemination. (Even if the original plan was to perform IVF, at this stage it might be determined that the plan should be changed to ICSI (Intra Cytoplasmic Sperm Injection) due to the recognition of a problem such as a low sperm mobility rate.) The fertilized eggs are then placed into a culture medium to be nourished while they are incubated.
5. Embroyo culturing
Approximately 18 hours after insemination, a microscope will be used to confirmed if and which eggs had been successfully fertilized. After confirmation the successfully fertilized eggs will be returned to the incubator. After 2 days each fertilized egg (embryo) will divide into 4 cells, and after 3 into 8 cells.
| Grade 1 | Grade 2 | Grade 3 | Grade 4 | Grade 5 |
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| Even blastomeres | Even blastomeres | Uneven blastomeres | Uneven blastomeres | Uneven blastomeres |
| no fragmentation | < 10% fragmentation | < 10% fragmentation | 10-50% fragmentation | > 50% fragmentation |
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